ABSTRACT
Yam tubers are prone to infestation by microorganism at all stages of growth and in storage. This rot reduces the quality of yam and affects food security. Pseudomonas fluorescens possess varieties of promising antifungal properties through production of secondary metabolites which makes it a biocontrol agent against many phyto-pathogens. In this study, the antifungal activities of metabolite produced by Pseudomonas fluorescens isolated in Zaria was evaluated. Rhizosphere soil was collected from different farms (sugarcane, rice and soybeans) in Zaria, Nigeria and Pseudomonas fluorescens was isolated and identified in accordance with standard microbiological procedure using cultural, microscopical and biochemical characteristics. The four isolate obtained were grown in three (3) fermentation media made from waste such as yam peel, banana peel and sugarcane shaft as carbon sources and groundnut cake as nitrogen source. Crude extract from these fermentations were tested for antifungal activities against Aspergillus niger, Penicillium citrinum, and Rhizopus stolonifer isolated from rotten yam using agar diffusion assay. Only four isolates labelled sugarcane A1, sugarcane A2, Rice B1 and Rice B2 were identified as Pseudomonas fluorescens and only fermentation broth extracts of Sugarcane A2, Rice B1 and Rice B2 had remarkable antifungal activities against the fungi with diameter zones of inhibition ranging from 14.00 – 39.00mm in two (2) of the fermentation media. The crude extracts from P. fluorescens fermentation broth were extracted in this study and were observed to inhibit the mycelial growth of the test fungi with zone of inhibition ranging from 19.50 - 33.50mm. The in vitro & in vivo MICs showed significant antifungal activities against test fungi spores. Rhizopus stolonifer was observed to be the most susceptible test organism in vitro & in vivo (0.15 – 5.00mg/ml). This study showed that the Pseudomonas fluorescens viii isolated in Zaria produced potential antifungal metabolite which can arrest the growth of phytopathogenic fungi associated with yam tubers.
1 Background Of The Study
This project attempts to examine the effect of strike on the academic perform...
ABSTRACT:- This work titled, “Implication of Local Government Autonomy on Rural Development in Ebonyi State” A st...
Background Of The Study
Throughout the history of philosophy, and especially in modern philosophy of c...
ABSTRACT
Forgery has long crept into our educational system and, counterfeit and forged certificate and document hav...
ABSTRACT
The El Nino Southern Oscillation (ENSO) has some level of control over the weather of Nigeria and Africa seasonally but there ha...
Abstract
This research discusses the impact of Christian religious studies on the morals of secondary school students in...
ABSTRACT
Comparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determ...
Background to the study
Socio-economic background or status refers to a person’s position in a gi...
Abstract
In the modern world, service oriented applications are becoming more and more popular from year to year. To remain competitive,...
ABSTRACT
In modern society, economic prosperity and progress depend largely on the quality of leadership. This is tr...